TABLE 2.
Oligonucleotide primers
| Primer | Sequence | Location relative to the first nucleotide of:
|
|
|---|---|---|---|
| folA | ydgB | ||
| eD5up | 5′ATTCTAGACGCCATGCTGTGGCTGATTGC | −1131 to −1111 | |
| eD3up | 5′GCGTCGACCGATAAAAAAAATTGTCGCC | −9 to −30 | |
| eD5down | 5′ATGTCGACTTTTGTATAGAATTTACGGC | +480 to +499 | |
| eD3down | 5′ATGCATGCGAAACCCCGCTGGGCACCATGC | +1519 to +1498 | |
| eD-short-up | 5′GTTTACGCTTTACGTATAGTGG | −50 to −31 | |
| eD-short-down | 5′GTCGCATCCGGCGCTAGCC | +515 to +497 | |
| ydgB5up | 5′ATCTAGAGGTGCATCGGCTTTATTGTGG | −1023 to −1003 | |
| ydgB3up | 5′GGATCCCGTTATCTCCTTTGCTATCCAACG | −1 to −36 | |
| ydgB5 down | 5′GGATCCTGGCGGTCGTCATCTGCG | +701 to +718 | |
| ydgB3 down SacI | 5′GAGCTCGGGTAGCTAATTCCCACAATAATTCG | +1708 to +1683 | |
| ydgB short up | 5′CGACCTGGATGCTGGTGGG | +59 to +41 | |
| ydgB short down | 5′CGGCATTGAAGCCTTACGCG | +905 to +996 | |