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. 2003 Dec;185(23):6790–6800. doi: 10.1128/JB.185.23.6790-6800.2003

FIG. 3.

FIG. 3.

Transformation of 3-chloromuconate by mixtures of MCI and trans-DLH of Pseudomonas sp. strain MT1. The reaction mixtures contained 50 mM Tris-HCl-2 mM MnCl2 (pH 7.5) and 120 to 140 μM 3-chloromuconate. The substrate and product concentrations were analyzed by HPLC. (A) Transformation catalyzed by 2.1 nM MCI. (B) Transformation catalyzed by a mixture of 0.7 nM MCI and 0.18 nM trans-DLH. (C) Transformation catalyzed by a mixture of 2.1 nM MCI and 5.4 nM trans-DLH. (D) Ratio of maleylacetate to protoanemonin formed from 3-chloromuconate by mixtures of MCI (2.1 nM) and various amounts of trans-DLH (0 to 102 nM).