TABLE 1.
Enzyme activities in cell extracts of Pseudomonas sp. strain MT1
| Enzyme | Substrate | Sp act (U/g of protein) after growth with:
|
||
|---|---|---|---|---|
| 5-Chlorosalicylate | Salicylate | Succinate | ||
| Salicylate 1-hydroxylase | Salicylate | 460 | 120 | <5 |
| 3-Chlorosalicylate | 120 | NDa | ND | |
| 4-Chlorosalicylate | 240 | ND | ND | |
| 5-Chlorosalicylate | 190 | ND | ND | |
| Catechol 2,3-dioxygenase | Catechol | <1 | <1 | ND |
| Catechol 1,2-dioxygenase | Catechol | 740 | 390 | <10 |
| 3-Chlorocatechol | 14 | <1 | ND | |
| 4-Chlorocatechol | 160 | 50 | ND | |
| MCI | Muconate | 35 | 370 | <5 |
| DLH | cis-Dienelactone | <5 | <5 | ND |
| trans-Dienelactone | 600 | 100 | <5 | |
| Protoanemonin | <5 | <5 | ND | |
| MAR | Maleylacetate | 1,120 | 510 | ND |
| cis-Acetylacrylate | 60 | 20 | ND | |
| trans-Acetylacrylate | <5 | <5 | <5 | |
a
ND, not determined.