TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′→3′)a |
|---|---|
| ptsI69-N | CCGTAAGCATATGACAGAAATGCTTAAA |
| pts1804R-X | GGCCTCGAGTTTTTAAATGATTAGTCCCTAC |
| IIA173 | TGAAGTACATATGGAATTGGAACATCGCTTTA |
| IIA173R | GTTCATCTCGAGTTCTCCTTTTTTGAAG |
| IIA-H91R-F | TTGTTCTTATCCGAGTTGGTATCGGAACAGTTAA |
| IIA-H91R-R | TTAACTGTTCCGATACCAACTCGGATAAGAACAA |
| manL44 | GGAGAACACATATGGGTATCGGTATTAT |
| manL1041R | ACTAATGGATCCGAATGAAGGTTATTGA |
| manH53F | GGAAGAACACATATGGGTATCGGTATTAT |
| manH1071R | AACTCATTTATGCATCCTCGAGAATTA |
a
Underlining indicates nucleotides participating in restriction sites. The nucleotides in boldface indicate the codon replacing His by Arg in IIALacS.