Skip to main content
. 2003 Dec;185(23):6985–6989. doi: 10.1128/JB.185.23.6985-6989.2003

FIG. 3.

FIG. 3.

Detection of Bam35-related genomes. (A) PCR amplification of Bam35-specific sequences from lysogenic cell lines and plaques with specific primers against Bam35c ORFs 6 and 14, producing PCR products of 235 bp and 810 bp, respectively. Lane 1 contains Generuler DNA ladder mix (MBI Fermentas). Lanes 2, 3, 5, and 7 contain PCR products obtained by colony PCR of host strain HER1410, lysogenic cell lines HER1410_L5 and HER1410_L7, and strain 4Q4, respectively. Lanes 4, 6, 8, and 9 show PCR products obtained by plaque PCR of cell lines HER1410_L5 and HER1410_L7, strain 4Q4, and a Bam35c plaque, respectively. Lane 10 contains the PCR products obtained from purified Bam35c DNA (control). (B) The lysogenic cell lines HER1410_L5 and HER1410_L7 and strain 4Q4 carry ≈15-kbp DNA molecules that cannot be found in the original Bam35 host strain HER1410. Lanes 1 and 2 contain purified PstI-digested λ DNA and purified Bam35c DNA, respectively. Lanes 3 to 6 contain purified DNA (Wizard Genomic DNA purification kit; Promega) from host strain HER1410, lysogenic cell lines HER1410_L5 and HER1410_L7, and strain 4Q4, respectively. The arrowhead depicts the ≈15-kbp DNA element found in the lysogenic cell lines and strain 4Q4.