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Expression of plasmid-encoded rRNAa
| Strain | Amt (%) of rRNA
|
|||
|---|---|---|---|---|
| 30S | 50S | 70S | 30S/50S ratio | |
| Δ7(prrnB) | 11 ± 1 | 29 ± 3 | 60 ± 5 | 0.38 |
| Δ7(pWD3) | 18 ± 1 | 30 ± 2 | 52 ± 3 | 0.60 |
| Δ7(pWD4) | 31 ± 1 | 29 ± 1 | 40 ± 1 | 1.07 |
| Δ7(pWD3) rnc | 20 ± 2 | 20 ± 1 | 60 ± 2 | 1.00 |
The relative cellular content of ribosomes was analyzed on linear 10 to 40% sucrose gradients. The percentage of each species was calculated as well as the 30S/50S ratio. Data shown are averages ± standard deviations for three separate determinations.
