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. Author manuscript; available in PMC: 2009 Sep 3.
Published in final edited form as: J Am Chem Soc. 2008 Aug 12;130(35):11614–11616. doi: 10.1021/ja8040042

Figure 2.

Figure 2

(a) Enoyl reduction is efficiently catalyzed by PksE(ER). (Left) PksJ-T1 active-site peptide. (Middle) PksJ-T2 active-site peptide. (Right) Ppant ejection ions. Vertical lines in (a) correspond to the most abundant isotopic peak (solid: absence of NADPH; dash: presence of NADPH). (b) PksC and PksE efficiently load several dihyrobacillaene-associated T domains (PksJ, PksL, AcpK) with 2-14C-malonate. C, including PksC; E, including PksE; -, no trans-AT. (Left) Coomassie-stained gel. (Right) Autoradiogram.