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. 2009 Jan 22;4(1):e4243. doi: 10.1371/journal.pone.0004243

Figure 3. Endogenous expression of LRP5Δ in MCF7 breast cancer cells is required for transcriptional activation of DKK1 by β-catenin.

Figure 3

(A) Transient cotransfections of DKK1 promoter luciferase constructs WT short or TBE3,4mut short [38] and CMV-LacZ to MCF7 cells (left panel). WT short, CMV-LacZ, and the indicated siRNAs were transfected (right panel). (B) Endogenous DKK1 mRNA expression in siRNA transfected MCF7 cells as indicated. (C) Transient cotransfections of TOPFLASH/FOPFLASH (left panel) or pTOPGlow/pFOPGlow (right panel) and CMV-LacZ to the indicated number of plated MCF7 cells.