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. 2009 Jan 22;4(1):e4243. doi: 10.1371/journal.pone.0004243

Figure 4. WNT3 ligand and LRP5Δ activate transcription in a DKK1 insensitive manner.

Figure 4

(A) MCF7 cells cotransfected with TOPFLASH, LRP5wt or LRP5Δ666–809 expression vectors, CMV-LacZ reference plasmid, followed by incubation in WNT1, WNT3 or WNT3A conditioned medium (CM). CM was from HEK293T cells transiently transfected with expression vectors for the various WNT and DKK1 ligands [26]. The 8-fold (Figure 2D) endogenous β-catenin activity is set to 1 (unstimulated, empty vector). (B) Cotransfection of TOPFLASH and CMV-LacZ reference plasmid to MCF7 cells (left panel) or HEK293T control cells (right panel). Incubation in WNT3 and DKK1 CM. HEK293T cells do not express the LRP5Δ receptor.