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. 2009 Jan 22;4(1):e4243. doi: 10.1371/journal.pone.0004243

Figure 5. Breast tumor cell growth is dependent on expression of LRP5Δ and continued β-catenin signaling by the receptor.

Figure 5

(A) Cell viability after transient siRNA transfection (24 h/48 h). Inhibition of cell growth was not seen in HeLa cells, that were used as control of toxic effects [26]. (B) Breast tumors from SCID mice injected with MCF7 cells pretransfected for 24 h with the indicated siRNAs. The animals were monitored every day and sacrificed after 5 weeks. The tumors were excised and weighed. (C) Endogenous β-catenin activity (left panel) and expression of LRP5wt and LRP5Δ as detected by immunoprecipitation and Western blot analysis (right panel) in T-47D breast cancer cells. (D) Effects on MCF7, T-47D and HeLa cell viability (96 h), non-phosphorylated active β-catenin level (MCF7), and transcription activation (24 h) by β-catenin (TOPFLASH) in the presence of the anti-LRP5 goat polyclonal antibody that recognizes both LRP5wt and LRP5Δ (Figure 1B, Figure 5C). Incubation without antibody or with normal goat serum showed similar effects (Control). (E) Detection of apoptosis during anti-LRP5 antibody incubation (96 h). Camptothecin treatment was used as positive control.