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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Neurovirol. 2008 Nov 12;14(5):376–388. doi: 10.1080/13550280802199898

Figure 2.

Figure 2

Quantitative immunohistochemical detection of phospho-JNK in macaque brain sections. (A) Staining for phospho-JNK in subcortical white matter was quantitated in samples from uninfected control animals (n = 4), SIV-infected animals (n = 6), and SIV-infected minocycline-treated animals (n = 5). Each data point represents the mean of 20 repeated measures of phospho-JNK staining in adjacent fields (bars represent group medians). Levels of active phospho-JNK in control animals were not significantly different from levels in SIV-infected untreated animals (p = 0.171; Mann-Whitney test), while SIV-infected minocycline-treated animals had significantly lower levels of phospho-JNK (p = 0.004; Mann-Whitney test). Staining for phospho-JNK with hematoxylin counterstaining to aid visualization (top) is shown in representative brain sections from an SIV-infected untreated animal (B) and an SIV-infected minocycline-treated animal (C). Representative staining present in subcortical white matter, without counterstain, as quantitated for (A) is shown at increased magnification (bottom). Prominent axonal staining is seen in untreated animals, and this is diminished in minocycline-treated animals. Original magnifications 100x and 200x.