Figure 3.

Quantitative immunohistochemical detection of nitrotyrosine in macaque brain sections. (A) Staining for nitrotyrosine in subcortical white matter was quantitated in samples from uninfected control animals (n = 6), SIV-infected untreated animals (n = 6), and SIV-infected minocycline-treated animals (n = 5) (bars represent group medians). Samples evaluated are serial sections of the same tissue from each animal stained for phospho-JNK in Figure 2. Each data point represents the mean of 20 repeated measures of nitrotyrosine staining in adjacent fields from one sample. Levels of nitrotyrosine staining were significantly increased from control at terminal infection (p = 0.009; Mann-Whitney test). SIV-infected minocycline-treated animals had significantly lower levels of nitrotyrosine immunopositive staining (p = 0.004; Mann-Whitney test). Staining for nitrotyrosine is shown in representative brain sections (top) from an untreated SIV-infected animal (B) and an SIV-infected minocycline-treated animal (C). Representative staining present in subcortical white matter, as quantitated in (A), is also shown at increased magnification (bottom). Original magnifications 100x and 200x.