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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Neurovirol. 2008 Nov 12;14(5):376–388. doi: 10.1080/13550280802199898

Figure 5.

Figure 5

Inhibition by minocycline of the activation of MKK3/6 and MKK7 in vitro. After four hours of treatment with 10 mM SNP, MKK3/6 and MKK7 were activated, as indicated by phospho-specific western blot analysis (representative blots shown). Pretreatment for four hours with minocycline prior to the addition of SNP led to a decrease in the activation of these MAPKKs. Western blot analyses for total MKK6 and MKK7 expression also are shown. Results are the means of four independent experiments, with error bars representing the SEM. Values illustrated are percent activation relative to activated samples (SNP treated, no minocycline). A one-sample t-test was performed to compare the mean activation level of each treatment group to 100%. Asterisks represent the lowest dose at which a statistically significant decrease in activation was seen compared to SNP stimulation (*p = 0.040, **p = 0.006); all higher doses also resulted in significant decreases.