Table 1.
S. agalactiae genes that are differentially regulated in the mtaR mutant.
| Locus (gene) | Function/annotation/homology | P value | Fold change |
| Transport and binding proteins | |||
| SAN_1753 (metP) | ABC transporter, permease | 4.4 × 10-6 | -2.43 |
| SAN_1754 (metN) | ABC transporter, ATP-binding | 5.8 × 10-7 | -2.43 |
| SAN_1756 (metQ) | ABC transporter, substrate-binding | 3.5 × 10-11 | -5.30 |
| SAN_0595 (artP) | ABC transporter, permease | 2.5 × 10-7 | -2.35 |
| SAN_0596 (artQ) | ABC transporter, ATP-binding | 9.7 × 10-6 | -2.44 |
| Protein fate: degradation of proteins, peptides, and glycopeptides | |||
| SAN_1755 (pdsM) | Peptidase, M20/M25/M40 family | 2.1 × 10-9 | -3.72 |
| SAN_2186 (cspA) | Serine protease | 2.3 × 10-7 | -3.20 |
| SAN_1255 | Streptokinase-like | 1.4 × 10-5 | -2.65 |
| Amino acid biosynthesis | |||
| SAN_0156 (argG) | Argininosuccinate synthase | 8.6 × 10-5 | -2.60 |
| SAN_0157 (argH) | Argininosuccinate lyase | 8.7 × 10-6 | -2.91 |
| Energy metabolism | |||
| SAN_0597 (manB) | Phosphomannomutase or Phosphoglucomutase | 4.1 × 10-5 | -2.04 |
| Hypothetical conserved | |||
| SAN_0933 | 8.3 × 10-4 | +2.47 | |
The differentially-regulated genes are grouped into functional categories, based on experimental evidence, protein database searches, and genome annotation. Each GBS COH1 gene [14] is indicated by its Comprehensive Microbial Resources locus number (SAN number) available on the J. Craig Venter Institute website http://www.jcvi.org/cms/research/projects/cmr/ and gene symbol, when applicable. Fold changes in gene expression (mtaR mutant relative to wild-type) and Bayesian P values were derived using Cyber-T [38] analysis of data from three independent biological replicates. Criteria for inclusion in this table were the following: Bayesian P value < 0.001, greater than two-fold change, and confirmation of differential expression by qPCR (Table 2).