Figure 6.

Expression of novel transcripts in microspore derived embryos following treatment with ABA. Early cotyledonary stage embryos were treated with 10 μM ABA (± racemic mix 1:1) in 0.1% ethanol or 0.1% ethanol without the hormone (Control) for 6 hours. Embryos were harvested, RNA extracted and analyzed for expression of a subset of novel genes identified from microarrays using k-PCR. All values represent the average of 2 biological replicates, each analyzed four times (technical replicates) ± SE.