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. 2009 Jan 30;5(1):e1000280. doi: 10.1371/journal.ppat.1000280

Figure 6. Rotavirus strains that express NSP1 induce proteasome-mediated degradation of β-TrCP.

Figure 6

(A) 293 cells were transfected with Flag-tagged β-TrCP (pFlag-βTrCP) and then infected with three pfu/cell of the indicated virus strain. Cell lysates were prepared eight hpi, and immunoblots were probed with anti-Flag antibody (top panel); anti-Flag and anti-NCDV NSP1 antibody (middle panel); or anti-Flag and anti-OSU NSP1 (bottom panel, Input). In the bottom right panel, the lysate from β-TrCP expressing, OSU infected cells was subjected to immunoprecipitation with anti-Flag M2 Agarose Affinity Resin, and blots were probed with anti-Flag antibody or anti-OSU NSP1 antibody (B) The same experiment as described in (A), except that proteasome inhibitor MG132 was added at the time of virus infection to final concentration of 10 µM.