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. 2007 Feb 20;48(1):48–54. doi: 10.3349/ymj.2007.48.1.48

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Copyright © 2007 The Yonsei University College of Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Immunohistochemical analysis of infiltrated stem cells in removed amniotic membrane. (A, B) Abundant CD34-positive cells, which were mainly composed of round or spindle-shaped mononuclear cells, were observed on the stromal side of the amniotic membrane (arrow). Occasionally, some CD34-positive cells formed clusters resembling small vessels (B; small arrow, inset). (C, D) c-kit immunoreactivity was found in grown epithelium (arrow head), spindle or round-shaped monocytes on the amniotic membrane (arrow). (E) SRTO-1-positive spindled-shaped cells resembling fibroblasts were observed (arrow). (F) Small round or long slender shaped AC133 positive cells (arrow). Sections were counterstained with hematoxylin. a, amnion side; s, amniotic membrane stroma. Original magnification: A, C 200×; B, B inset, D, E 400×. A, C: cross-section, B, D, E, F: flat mount.