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Western blot analysis of 15-PGDH and PCNA in gastric carcinomas. 15-PGDH expression was inversely correlated with PCNA expression in gastric carcinomas (p < 0.05, R = - 0.55). Forty µg of protein was separated by 10% SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane. The bottom represents β-actin, which was used as a loading control. 15-PGDH, 15-hydroxyprostaglandin dehydrogenase; PCNA, proliferating cell nuclear antigen.
