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Lack of consistency of housekeeping gene expression between treatment groups and among donors. A: Differences between treatment and untreated control in RT-PCR amplification replicates from a single donor’s CD4+ T cells and monocytes. CD4+ T cells were treated with PMA and ionomycin; monocytes were treated with lipopolysaccharide. B: Relative expression of RPL13A, HMBS, and YWHAZ in untreated CD4+ T cells or monocytes from 6 donors.
