FIGURE 6.
Electroporation of Neuro-2a cells using an exponential waveform pulse of 250 V, 350 μF, and 1000 Ω. Cells were assayed 24 h after transfection. A: Cells analyzed by flow cytometry. Nonelectroporated negative control cells (top) and electroporated cells (bottom) were treated with fluorescent TC siRNA in electroporation buffer. We used 4 × 106 cells/mL in these experiments. B: Cells analyzed by microscopy. Cells were electroporated with pEGFP plasmid DNA. Top: Bright field image of Neuro-2A cells. Bottom: fluorescent image. We used 2 × 106 cells/mL in these electroporations.