Table I.

The DNA sequences of the primers and probes for real-time RT-PCR experiments

Expression of CGB gene transcripts
Real-time primer	Fig.1 Label	Sequence with Fluorescent Dye	Tm (°C)	Product size
CGB1/2F	A	5′-AACACCCCTCACTCCCTGTCT-3′	58.1	139 bp
CGB1/2R	B	5′-ATGCTCAGCAGCAGCAACA-3′	57.8
alt47C1majR	C	5′-GCAGCAGCCTCTGGAACATCT-3′	58.6	167/ 165 bp
alt47C1min/C2R	D	5′-AGCAGCCCCTGGAACATCTC-3′	58.3
alt176C1/C2intF	E	5′-GCCATCACTGGCATGAGAAG-3′	57.8	96 bp
alt176C1majR	F	5′-AGCAGCAGCCTTGAAGCTTACT-3′	58.6
alt176C1min/C2R	G	5′-AGCAGCAGCCCTGAAGCTTAC-3′	59.8
CGBallF	H	5′-TCACCGTCAACACCACCATCT-3′	59.8	121 bp
CGBtotalR	I	5′-ATGGACTCGAAGCGCACATC-3′	59.8
Taqman probe
CGB1A	1	5′-FAM-ACATGTCAAAGAGGCTG-MGB-3′	69.2
CGB1G	2	5′-VIC-CATGTCAAAGGGGCTG-MGB-3′	69.9
CGB1 †	3	5′-FAM-CATGTCCACATTCCCAGTG-MGB-3′	68.2
CGB2†	4	5′-FAM-CATGTCCACATCCCCAGT-MGB-3′	67.9
alt176C1/2	5	5′-FAM-CTCTTTCTGGAGGAGCGT-MGB-3′	68.3
CGBall	6	5′-FAM-CCACCATGACCCGCGT-MGB-3′	68.6
Primers for plasmid construction
CGB8F		5′-AGCACCTTTCTCGGGTCAC-3′	60.2	791 bp
CGB8R		5′-GGCCTTTGAGGAAGAGGAGT-3′	59.5
CGB1F		5′-GAGGAAGGGGAACTGCATCT-3′	60.6	686/ 689 bp
CGB2F		5′-AGGGAGGAAGGGGAACTGTA-3′	59.9
CGB1, CGB2R		5′-TGCGGATTGAGAAGCCTTTA-3′	60.8
CGB1Fnested		5′-GAAGGGGAACTGCATCTGAG-3′	59.8	406 bp*
CGB1Rnested		5′-GGTAGTTGCACACCACCTGA-3′	59

Tm, melting temperature; F, forward primer; R, reverse primer;

^†the probe was used to detect CGB1/CGB2 + 47 bp alternative mRNA form

^*the primers amplified CGB1 + 176 bp mRNA form, 581 bp and CGB1 + 166 bp mRNA alternative form, 571 bp.