Table I.
Molecular Masses Determined by MALDI-TOF-MS of sCT, Lys11-mono-bio-sCT and Lys11,18-di-bio-sCT Before/After Lys-C Digestion
| Calculated mass (m/za) | Observed masses (m/za) | |
|---|---|---|
| Before Lys-C digestion | ||
| Product | ||
| sCT | 3,432 | 3,422.21 |
| Lys11-mono-bio-sCT | 3,676 | 3,660.5 |
| Lys11,18-di-bio-sCT | 3,920 | 3,900.2 |
| After Lys-C digestion | ||
| Possible fragments | ||
| Cys1-Lys11 | 1,106.34 | 1,128.04 |
| Leu12-Lys18 | 835.96 | 848.02 |
| Leu19-Pro32 | 1,475.61 | 1,492.9 and 1,495.22 |
| Leu12-Lys18-bio-Pro32 | 2,555.57 | 2,208.87 |
| Cys1-Lys11-bio-Lys18 | 2,186.3 | – |
amass/charge