Table 1.

Peptides identified from LC-ES-MS/MS analysis of CNBr cleavage followed by trypsin digestion of the precipitated NTS1 receptor. Letters in bold correspond to TM domains. Overlapping peptides are not shown. Formyl = formylation, Hse_lact = homoserine lactone, ox = oxidation and * = carbamidomethylation.

Peptide sequence	Residue number	Experimental mass (m/z)	Theoretical mass (m/z)	Mass Accuracy (ppm)	Charge state
SGSTSESDTAGPNSDLDVNTDIYSK	1–25	1280.568	1280.562	4.3	2+
VLVTAIYLALFVVGTVGNSformylVTAFTLAR	26–52	941.877	941.872	4.7	3+
SLQ SLQSTVHY	55–65	631.824	631.822	2.0	2+
LLILLLAMHse_lact	75–82	851.600	851.597	3.9	1+
PVELYNFIWVHHPWAFGDAGC*R	83–104	668.574	668.572	2.8	4+
GYYFLR	105–110	409.713	409.713	0	2+
DAC*TYATALNVASLSVER	111–128	970.977	970.972	4.3	2+
DAC*TYATALNVASLSVERYLAIC*HPFK	111–137	1024.181	1024.176	5.0	3+
YLAIC*HPFK	129–137	574.800	574.800	0	2+
LASALLAIPMoxLFTMoxGLQNR	156–174	1046.572	1046.568	3.8	2+
LFTMoxGLQNR	166–174	548.285	548.285	0	2+
SGDGTHPGGLVC*TPIVDTATVK	175–196	728.033	728.032	2.1	3+
VVIQVNTFMHse_lact	197–205	1002.563	1002.562	1.3	1+
SFLFPMHse_lact	206–211	693.362	693.361	2.5	1+
LVISILNTVIANK	212–224	699.442	699.440	3.0	2+
LTVMVHQAAEQGR	225–237	720.376	720.375	1.4	2+
VC*TVGTHNGLEHSTFNMHse_lact	238–254	928.425	928.423	2.4	2+
VC*TVGTHNGLEHSTFNMoxTIEPGR	238–260	858.402	858.401	1.8	3+
HGVLVLR	266–272	397.256	397.256	0	2+
AVVIAFVVC*WoxLPYHVR	273–288	973.034	973.029	4.5	2+
FYHYFYMHse_lact	307–313	511.725	511.724	1.9	2+
LTNALFYVSSAINPILYNLVSANFR	314–338	1400.763	1400.755	5.3	2+
QVFLSTLAC*LC*PGWR	339–353	904.457	904.453	4.5	2+
KformylRPTFSR	358–364	307.175	307.175	0	3+
KPNSMoxSSNHAFSTSATR	365–381	613.620	613.620	0	3+
SformylSNHAFSTSATR	370–381	647.295	647.295	0	2+
ENLYFQ	382–387	813.378	813.378	0	1+