Table 1.
Hairpin mutants of human RECQ1 and E.coli RecQ
| Mutant | Hairpin sequence |
|---|---|
| Human RECQ1 | |
| RECQ1T1 | 554YLKEDYSFTAYATISYLKIG573 |
| Y564A RECQ1T1: | 554YLKEDYSFTAAATISYLKIG573 |
| Δ2-Y564A RECQ1T1: | 554YLKEDYS---AA----ISYLKIG573 |
| Δ3-Y564A RECQ1T1: | 554YLKEDY-----AA----SYLKIG573 |
| Δ8-Y564A RECQ1T1: | 554Y--------------AA------------G573 |
| E. coli RecQ | |
| RecQ and RecQΔC | 484VTQNIAQHSALQL496 |
| H491A RecQ and H491A RecQΔC | 484VTQNIAQASALQL496 |
| Δ3 RecQ and Δ3 RecQΔC | 484VTQNIA—ALQL496 |
The E.coli RecQ proteins denoted ΔC encompass amino acids 1-524 and lack the C-terminal HRDC domain. The strands of the β-hairpins are underlined.