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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Am Chem Soc. 2008 Sep 18;130(41):13673–13682. doi: 10.1021/ja803612z

Figure 4.

Figure 4

(a) dependence of kcat/KM on pH for the hydrolysis of 4NPP (open circles) and 4NPMP (closed circles) catalyzed by PP1γ. Solid lines are fits to equation 1 as described in the text. (b) Inhibition by inorganic phosphate of 4NPP hydrolysis (open circles) and 4NPMP (closed circles) catalyzed by PP1γ at pH 8. Solid lines are fits to equation 2 as described in the text. Conditions: 25 °C, 100 mM buffer, 1 mM MnCl2 (I = 0.45 M (NaCl)).