Table 1.

Morphometric analysis of nuclei of basal epithelial cells

	Nuclear area (μm2)	Major axis length (μm)	Minor axis length (μm)	Eccentricity
Conjunctiva	82 ± 27	12.5 ± 2.9	8.7 ± 1.6	0.67 ± 0.15
Limbus	65 ± 24	11.8 ± 2.9	7.3 ± 1.4	0.74 ± 0.14
Cornea	45 ± 16	9.4 ± 2.1	6.2 ± 1.3	0.71 ± 0.15

4′,6-diamidino-2-phenylindole–stained nuclei in a flat whole mount were imaged and analyzed with CellProfiler. Average and SD are shown. The number of nuclei scored for the analysis was 133, 173, and 786 for conjunctiva, limbus, and cornea, respectively. Eccentricity is a ratio of the distance between the foci of the ellipse and its major axis length. A t-test showed that the data between conjunctiva, limbus, and cornea were significantly different from each other in all four data sets with criteria of p<0.05.