Table 2.
FRET analysis
| Age (days) | FRET | Odontoblasts | Predentin | Dentin | Proliferation | Hypertrophy | Ossification zone |
|---|---|---|---|---|---|---|---|
| 1 | D-pre | 100.7 | 74.8 | 107.7 | 193 | 127.3 | 63.7 |
| D-post | 112.6 | 73.0 | 107.3 | 189.9 | 130.2 | 59.3 | |
| A-pre | 198.8 | 132.2 | 255 | 74.8 | 226.1 | 72.4 | |
| A-post | 152.1 | 106.6 | 254.9 | 73.1 | 214.5 | 64.4 | |
| Eff. | 10 | 0 | 0 | 0 | 2.7 | 0 | |
| 7 | D-pre | 8.5 | 24.3 | 3.9 | 30.3 | 18.4 | 27.5 |
| D-post | 9.1 | 23.6 | 3.5 | 30.1 | 19.4 | 27.8 | |
| A-pre | 44 | 30.5 | 62.3 | 0.9 | 37.3 | 47 | |
| A-post | 42.3 | 30 | 60 | 0.6 | 35.3 | 46.1 | |
| Eff. | 6.3 | 0.5 | 0 | 0 | 5.2 | 1 | |
| 21 | D-pre | 46 | 54.4 | 13.7 | 53 | 210.3 | 30.6 |
| D-post | 48.6 | 54.3 | 11.7 | 52.4 | 205.6 | 30.3 | |
| A-pre | 203.5 | 113.4 | 183.5 | 115.9 | 130.3 | 42.8 | |
| A-post | 176.4 | 92.1 | 116 | 102.6 | 113.7 | 40 | |
| Eff. | 6.5 | 0.5 | 0 | 0 | 0 | 0 | |
| 35 | D-pre | 72.9 | 95.6 | 219.1 | 139.6 | 67.2 | 68.8 |
| D-post | 77.3 | 96.4 | 213.3 | 139.7 | 68.7 | 67.9 | |
| A-pre | 92.2 | 110.7 | 130.4 | 3.6 | 38.7 | 138.3 | |
| A-post | 58.8 | 99.8 | 119.5 | 3.6 | 38.1 | 133.2 | |
| Eff. | 5.4 | 1.6 | 0 | 0 | 3.5 | 0 | |
| 56 | D-pre | 49 | 105.3 | 23.1 | 120.6 | 189.4 | 70.7 |
| D-post | 50 | 104.7 | 22.6 | 116.9 | 184.3 | 67.3 | |
| A-pre | 131.4 | 15.8 | 33.1 | 0.5 | 14.6 | 96.0 | |
| A-post | 131 | 14.2 | 32.5 | 0.3 | 11.1 | 95.4 | |
| Eff. | 2 | 0.5 | 0 | 0 | 0 | 0 |
The mean FRET efficiency (Eff), calculated on the basis of acceptor photobleaching method; donor fluorescence emission before (D-pre) and after (D-post) acceptor photobleaching, acceptor fluorescence emission before (A-pre) and after (A-post) acceptor photobleaching. Mean was calculated with Excel Microsoft on the basis of rough data provided directly with the photobleaching reports by software (Leica). Eff = 0 means there was no FRET; Eff = >1 means FRET occurred between fluorescent probes.