Figure 7.

Expression of miR-15a and c-Myb in human CD34⁺ cells cultured under conditions favoring erythroid differentiation. Human CD34⁺ cells were grown in medium supplemented with SCF and Epo. The expression of miR-15a (A) and c-myb (B) was examined on days 1, 2, 3, 4, 6, 8, and 10 using mirVana qRT-PCR miRNA detection method for miR-15a, or general real-time PCR for c-myb. c-Myb protein was measured by western-blotting (C) and quantitation of the c-Myb protein was done by density analysis (D). Representative data from 3 independent experiments are shown.