Figure 5.

Analysis of CD8⁺ T cells infiltrating rAAVeGFP and rAdeGFP-transduced quadriceps muscle. (A) H-2K^d eGFP_200-208 tetramer staining of lymphocytes isolated from spleen, liver, and muscle of mice injected with rAAVeGFP and rAdeGFP 56 days earlier. Numbers represent the percentage of CD8⁺ T cells that costain with the eGFP tetramer. Plots were gated as in Figure 1D. (B) Annexin V binding by H-2K^d eGFP_200-208 tetramer⁺ CD8⁺ T lymphocytes harvested from quadriceps muscle 56 days after transduction with rAAVeGFP or rAdeGFP. Annexin V binding by eGFP_200-208-specific CD8⁺ T cells isolated from the spleen and liver of the same animals are shown for comparison. Plots gated as in Figure 4A (right column). Numbers represent the percentage of tetramer⁺ cells (rAAVeGFP and rAdeGFP-inoculated animals) that bind annexin V. Data represent 4 independent experiments each with tissues pooled from 5 mice. For interpretation of differences in annexin V binding by tetramer⁺ cells in spleen, liver and muscle, an analysis of variance test was performed (1-way ANOVA for correlated samples followed by Tukey's test). For the rAAVeGFP-treated mice, spleen versus muscle P < .01, liver versus muscle P < .01 and spleen versus liver P > .1. For the rAdeGFP-treated mice, differences in variance among the 3 tissues were not significant.