Table 2.

16S ribosomal gene primers used for PCR amplification or DGGE

Primer	Sequence (5'-3')	Primer target sitea	Reference
Amp F	GAGAGTTTGATYCTGGCTCAG	0006	[13]
Amp R	AAGGAGGTGATCCARCCGCA	1530	[13]
InfL6b	TATCGGGGAGCAAGCGAG	0445	[53]
Bif662-r	CCACCGTTACACCGGGAA	0662	[3]
ENT183c	CGTCGCAAGACCAAAGAG	0183	[54]
424R	CCGCTGAAAGTACTTTACAACC	0424	This study
F-968d	GAACGCGAAGAACCTTAC	0968	[55]
R-1401	CGGTGTGTAGAAGACCC	1401	[55]
F-341d	CCTACGGGAGGCAGCAG	0341	[56]
R-534	ATTACCGCGGCTGCTGG	0534	[56]

^a Primer target site according to E. coli 16S ribosomal gene numbering as the reference.

^b This primer was known as B. longum grp3 for the microarray.

^c This primer was designated Enterobacteriaceae1 for the microarray.

^d A 39 nt GC clamp was added to the 5' end of the primer when used for DGGE.