Figure 6.
Hippocampal regulation of GluR1S845 and ERK phosphorylation in the (A) CA1 and (B) CA3 subregions during spontaneous WD from chronic intermittent heroin self-administration. Microwave-fixed brain tissue was collected after 12 or 24 hrs spontaneous WD, or 1 hr following an injection of 1 mg/kg naltrexone in drug-naïve (NTX) and 24 hr WD (24 hr + NTX) animals. Data are presented as the mean ± SEM of phosphorylated (p)/total protein ratios expressed as a percent change from untreated age and batch-matched controls (n = 8−11/group). Asterisks indicate values differ from 12 hr WD group (*p < 0.05, **p < 0.01) by Newman-Keuls posthoc tests. Representative blots for phosphorylated and total GluR1 and ERK protein are shown.