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. 2008 Aug 29;70(2):396–409. doi: 10.1111/j.1365-2958.2008.06418.x

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Journal compilation © 2008 Blackwell Publishing Ltd No claim to original US government works

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Fig. 4 — The TroR_Td repressor responds to Mn²⁺ and Fe²⁺ in E. coli. (A) Strain DEN113 expressing pTDE100 (T. denticola troP/O-lacZ reporter) and pPJB113 (expresses TroR_Td) or (B) strain PAL113 expressing pPAL100 (T. pallidum troP/O-lacZ reporter) and pPJB113 (expresses TroR_Td) were grown aerobically overnight in M9CG media supplemented with various divalent cations (5 μM) or in unsupplemented M9CG media (control). β-Galactosidase activity of the overnight cultures was determined as described by Miller (1972). Results represent the means and standard deviations of three independent experiments.