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. 2008 Aug 15;70(1):76–88. doi: 10.1111/j.1365-2958.2008.06389.x

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© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd

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Fig. 5 — LuxR binds and regulates qrgB.

A. The consensus PWM for the LuxR binding site is shown for comparison with the actual binding site in the qrgB promoter. The wild-type binding site is scored at 0.65 (dashed line). The average SVM score for the three substitutions at each base is presented versus the location in the binding site. Error bars indicate the standard deviation of the mean score for mutations at each position.

B. In vivo repression of qrgB–gfp expression is shown for the wild-type promoter, single- and double-point mutants. Measurements were made in triplicate, and fold repression calculated as the ratio LuxR^-/LuxR⁺. Error bars represent the standard deviation of the ratio, calculated via the formula for propagation of error.

C. DNA binding curves for LuxR binding to the wild-type qrgB binding site (red), qrgB A2C (light blue), qrgB A6C (green), qrgB A17C (dark blue) and qrgB A2C, A17C (purple). The fractional change in anisotropy is plotted against the concentration of LuxR (nM). Error bars represent the standard deviation of three independent binding reactions.