Skip to main content
. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Int J Biochem Cell Biol. 2008 Jul 4;41(3):546–553. doi: 10.1016/j.biocel.2008.06.007

Figure 3. Mapping of the regulatory elements in the LRAT promoter.

Figure 3

(A) Reporter constructs resulting from deletions of the LRAT promoter 5′-flanking region were prepared as described in Materials and Methods. (B) PrEC and PC-3 cells were transiently transfected with pLRAT2008 (3.1 μg), pLRAT833 (2.5 μg), pLRAT478 (2.4 μg), pLRAT186 (2.3 μg), pLRAT14 (2.2 μg), pGL 3-basic plasmid (negative control; 2.2 μg), or pCYP26-Luc (positive control; 2.2 μg), together with pRL-TK (0.8 μg) for normalization of transfection efficiency. Then cells were cultured in the medium with 0.1% ethanol (control), or 1 μM RA for 48 h. Luciferase activities were measured by a dual luciferase reporter assay system (Promega), and expressed relative to the value derived from PrEC cells transiently transfected with the pLRAT2008 and treated with 0.1% ethanol (control) for 48 h. The values were obtained from three independent experiments. *, p <0.05; * *, p <0.01 (compared to control).