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. Author manuscript; available in PMC: 2009 Oct 25.
Published in final edited form as: Virology. 2008 Sep 6;380(2):338–343. doi: 10.1016/j.virol.2008.07.029

Figure 5. JAK1 and STAT2 but not STAT1 are necessary for induction of ADAR1 expression by interferon alpha.

Figure 5

Figure 5

Western immunoblot analysis of ADAR1 p150 protein expression in WT and mutant MEF cell lines. Cells were either treated with IFN-αA/D (+) or left untreated (−). Nonidet P-40 cell free extracts were prepared and ~60 µg protein was analyzed by Western immunoblotting as described under Materials and Methods. (A) Cell-free extracts prepared from WT and mutant MEF cells genetically deficient in Sp3, Stat1, Jak1 or the IFNAR receptor (Rc) as indicated. (B) Cell-free extracts prepared from WT and mutant MEF cell lines genetically deficient in Stat1 or Stat2.