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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Immunol. 2008 Sep 15;181(6):4371–4380. doi: 10.4049/jimmunol.181.6.4371

Figure 6. Tolerance can be assessed by early chimerism and cytotoxicity assays 7–8 days after BMT.

Figure 6

(A) B6 mice received either conditioning with TBI and anti-CD154 mAb or with TBI only without anti-CD154. They were then transplanted with BMCs from fully MHC-mismatched B10.A mice. The percentage of donor B cells and granulocytes in the spleen is shown on day 7 after BMT. (B) B6 mice received conditioning with TBI and anti-CD154 and were then transplanted with BMCs from fully MHC-mismatched B10.A mice. Cytotoxic activity of whole splenocytes on day 8 after BMT was measured in a 51Cr release assay against donor B10.A (closed symbols) or 3rd party B10.RIII targets (open symbols) after a 5 day restimulation culture with the respective stimulators (mean ± SEM, 4 mice/group). One representative experiment out of three is shown.