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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Vaccine. 2008 Oct 1;26(50):6329–6337. doi: 10.1016/j.vaccine.2008.09.031

Figure 8.

Figure 8

CD4+ cells are required at time of challenge for complete protection against plague challenge. (A) Timeline for the depletion-challenge study. Mice in four groups (A-D) were immunized IM with 109 pfu of VSV-ssLcrV1. Serum sampling was done on day 28, 56 and 82 post immunization. Mice were injected with CD4+/CD8+ depleting MAbs (IP) on days 90 and 91 post immunization followed by challenge on day 91 with 10 LD50 (10,000 cfu) of Y. pestis (CO92 strain). VSV-eGFP1 vaccinated mice were used as the control group. Rat IgG2b isotype MAb was used as a control. (B) Survival profile for mice in the depletion-challenge experiment. VSV-ssLcrV1 vaccinated mice were injected with isotype-matched control (filled square), anti-mCD4 (filled triangle), anti-mCD8 (open diamond) or mCD4+mCD8 (open circle) MAbs. Control animals receiving VSV-eGFP1 (open triangle) were not treated with MAbs.