Primers used for library screening and junction sequencing |
MT85.1 |
ACAGTAATTGCGGGTGGATC |
60 |
MT85.2 |
TTACCGCCTTTGAGTGAGCT |
60 |
GlpO6′ |
GCTCTTGCACTTGTTGTTCC |
60 |
GlpO7 |
ACAGATTGCCACTGCTCATTT |
60 |
GtsA3 |
ATGGATCCGCATGCTAGGACCTTCTGGGAGTGGA |
62 |
GtsB3 |
GCTCAGCTGCCGACGCTCTAATAAGGCTGCTGCAAATAAAAT |
58 |
GtsB1 |
GATCGCATGCAAGATTTGCACCAAATGAAGC |
58 |
GtsC4 |
ATCTGCAGGCATGCCATAGCACCAGCTGCTTGAA |
58 |
LppQ1 |
GATCGCATGCAAAAATGAGTGACAAAAACAAGAT |
60 |
LppQ2 |
ATATGCATGCTGTGTCAACCAAAGGCATC |
58 |
Primers used for the construction of pCJ13, pCJ15 and pMT85/2res |
LppQ7 |
ATATGCATGCTGGTGTTGCTACTGAAATTTGT |
60 |
LppQ8 |
ATATGCATGCCTGTTCCTCTTGCCCATCAT |
60 |
PSresR |
ATATGCATGCATTAAGCAATTTATTTGGAAAATC |
56 |
PSresL |
ATATGCATGCTTAGTTGCTTTCATTTATTACTT |
56 |
res5 |
ATATACTAGTGGATCTCATAAAAATGTATCCTA |
60 |
res6 |
ATATGGATCCGCAACCGTCCGAAATATTATAA |
60 |
res7 |
ATATGCTAGCGGATCTCATAAAAATGTATCCTA |
60 |
res8 |
ATATGGTACCGCAACCGTCCGAAATATTATAA |
60 |