Skip to main content
. Author manuscript; available in PMC: 2009 Jan 19.
Published in final edited form as: Microbiology (Reading). 2008 Aug;154(Pt 8):2427–2436. doi: 10.1099/mic.0.2008/017640-0

Table 1.

Primers used in this study

Primer Sequence 5′ -3′ Tm (°C)
Primers used for library screening and junction sequencing
MT85.1 ACAGTAATTGCGGGTGGATC 60
MT85.2 TTACCGCCTTTGAGTGAGCT 60
GlpO6′ GCTCTTGCACTTGTTGTTCC 60
GlpO7 ACAGATTGCCACTGCTCATTT 60
GtsA3 ATGGATCCGCATGCTAGGACCTTCTGGGAGTGGA 62
GtsB3 GCTCAGCTGCCGACGCTCTAATAAGGCTGCTGCAAATAAAAT 58
GtsB1 GATCGCATGCAAGATTTGCACCAAATGAAGC 58
GtsC4 ATCTGCAGGCATGCCATAGCACCAGCTGCTTGAA 58
LppQ1 GATCGCATGCAAAAATGAGTGACAAAAACAAGAT 60
LppQ2 ATATGCATGCTGTGTCAACCAAAGGCATC 58
Primers used for the construction of pCJ13, pCJ15 and pMT85/2res
LppQ7 ATATGCATGCTGGTGTTGCTACTGAAATTTGT 60
LppQ8 ATATGCATGCCTGTTCCTCTTGCCCATCAT 60
PSresR ATATGCATGCATTAAGCAATTTATTTGGAAAATC 56
PSresL ATATGCATGCTTAGTTGCTTTCATTTATTACTT 56
res5 ATATACTAGTGGATCTCATAAAAATGTATCCTA 60
res6 ATATGGATCCGCAACCGTCCGAAATATTATAA 60
res7 ATATGCTAGCGGATCTCATAAAAATGTATCCTA 60
res8 ATATGGTACCGCAACCGTCCGAAATATTATAA 60