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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: FEBS Lett. 2008 Nov 4;582(29):4039–4046. doi: 10.1016/j.febslet.2008.10.043

Figure 4.

Figure 4

Effects of blocking GPCR activation in the presence of inflammatory mediators. (A-D) Immunofluorescence images of Cx43-based GJs showed that neither GJ internalization nor cell separation occurred in PAECs treated with 240nM suramin alone (A), or with suramin plus 50nM endothelin-1 (B), 10U/mL thrombin (C), or 240nM mastoparan (D) for 30 min. (E-H) GJIC was assessed by scrape loading dye transfer in PAECs treated under the same conditions as in A-D. (I-L) Linescans of average fluorescence intensity measured along the lines indicated in E-H.