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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: Cancer Res. 2008 Nov 15;68(22):9441–9450. doi: 10.1158/0008-5472.CAN-08-0900

Figure 4. TIL isolated from a patient with metastatic melanoma secrete predominantly TH2-type cytokines.

Figure 4

A. Tumor infiltrating lymphocytes (TIL) were isolated from a resected melanoma, expanded in the presence of IL-2, and frozen for storage in liquid nitrogen. Thawed TIL were rested for 24 hrs in the absence of any cytokines. Upon stimulation with PMA plus Ionomycin for 6 hours, both CD8+ and CD4+ T cells secreted high levels of the IL-4 and little or no IFN-γ. T cells were tested by ICS. Results are representative of 3 separate experiments.

B. TIL were stimulated as above with PMA plus Ionomycin and lysed. mRNA was extracted and subjected to RT-PCR. cDNA for 49 different T cell products was measured by qPCR, and values were normalized to the quantity of actin mRNA. Values shown represent those factors for which PMA treatment resulted in >5 X induction of specific message.