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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: Cancer Res. 2008 Nov 15;68(22):9441–9450. doi: 10.1158/0008-5472.CAN-08-0900

Figure 6. IL-12-transfected DC enhance priming of MART-1-specific TH1 like cells.

Figure 6

A. MDDC from HLA A*0201 melanoma patients were transfected with LysoMART-1 mRNA only or with LysoMART-1 plus IL-12p70 mRNA and used to prime autologous T cells. T cells lines from two patients shown were stimulated using 2 μM of the HLA A*0201 restricted MART-1(21-35) peptide in the presence of BFA for 6 hours and IFN-γ production was determined by ICS.

B. Culture supernatants of T cell lines were evaluated for cytokine secretion following overnight stimulation in the absence or presence of 2 μM MART-1(21-35) peptide.