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. 2008 Dec 31;28(53):14329–14340. doi: 10.1523/JNEUROSCI.4833-08.2008

Figure 2.

Figure 2.

Localization of the Nav1.6 subunit in AISs in layer 2/3 of the neocortex. A, The identical immunolabeling of AISs and nodes of Ranvier with a rabbit (Nav1.6-R) and a mouse (Nav1.6-M) anti-Nav1.6 antibody, raised against different, nonoverlapping epitopes, demonstrates the specificity of the immunolabeling. B, C, Double immunofluorescent reactions reveal the colocalization of the Nav1.6 subunit with AIS markers Ankyrin-G (B) and Neurofascin (C). Note the proximo-distal gradient of the Nav1.6 subunit labeling in contrast to the even distribution of Ankyrin-G (B, inset) and Neurofascin (C) along the AISs. Scale bars, 10 μm.