FIG. 2.

Ectopic overexpression of SIRT1 regulates HDAC activity in RIN cells. A: RIN cells (5 × 10⁶) were transduced with retroviruses expressing WT-SIRT1 or DN-SIRT1, and RIN cell extracts prepared 48 h after viral infection were subjected to Western blot analysis with anti-SIRT1 antibody. B: Deacetylase activity of overexpressed SIRT1 was measured using fluorogenic substrate as described in research design and methods. Each value represents the mean ± SE of three independent experiments. **P < 0.01 vs. pBABE.