FIG. 3.

Effects of SIRT1 overexpression on cytokine-induced cytotoxicity. Control or SIRT1-overexpressing RIN cells (1 × 10⁵) were treated with IL-1β alone or IL-1β and IFN-γ for 48 h. The cell viability (A) and cell proliferative potential (B) were then determined using an MTT and BrdU incorporation assay, respectively. Each value represents the mean ± SE of three independent experiments. *P < 0.05, **P < 0.01 vs. untreated pBABE; ##P < 0.01 vs. IL-1β + IFN-γ–treated pBABE; $$P < 0.01 vs. untreated DN-SIRT1.