FIG. 5.

Effect of SIRT1 overexpression on cytokine-induced NF-κB activation and translocation and acetylation of p65. Control or SIRT1-overexpressing RIN cells were treated with IL-1β alone or IL-1β and IFN-γ. After 1 h of incubation, DNA binding (A) and transcriptional (B) activities of NF-κB were analyzed by EMSA and luciferase reporter assay, respectively. C: The nuclear translocation of p65 and acetylation of p65 at K310 were determined by Western blotting. PCNA was used as loading control for nuclear protein. Each value represents the mean ± SE of three independent experiments. *P < 0.05, **P < 0.01 vs. untreated pBABE; ##P < 0.01 vs. IL-1β + IFN-γ–treated pBABE; $$P < 0.01 vs. untreated DN-SIRT1. PCNA, proliferating cell nuclear antigen; RLU, relative light unit.