FIG. 6.

Inhibition of cytokine-induced activation of the NF-κB pathway by resveratrol in RIN cells. RIN cells were treated with IL-1β and IFN-γ with or without a 3-h pretreatment with resveratrol. SIRT1 deacetylase activity was determined 1 h later (A), and nitrite production and iNOS mRNA and protein expressions were determined 24 h later (C). Transcriptional activity of NF-κB (B) and acetylation of p65 (D) were analyzed by luciferase reporter assay and Western blotting, respectively. Each value represents the mean ± SE of three independent experiments. **P < 0.01 vs. untreated control; ##P < 0.01 vs. IL-1β + IFN-γ.