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. 2009 Feb;58(2):377–384. doi: 10.2337/db08-0303

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Copyright © 2009, American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — The expression profiles and intracellular localization of DOC2b in 3T3-L1 adipocytes. A: The expressions of DOC2a and DOC2b in 3T3-L1 fibroblasts, 3T3-L1 adipocytes (days 3 and 9 of differentiation), rat epididymal fat, and mouse brain were analyzed by mRNA blot. B: Differentiated 3T3-L1 adipocytes were serum-starved for 3–4 h and then stimulated with 100 nmol/l of insulin for 20 min, fixed, and immunostained. The endogenous DOC2b and GLUT4 were visualized using anti-DOC2b and anti-GLUT4 antibodies and observed by confocal microscopy. (Please see http://dx.doi.org/10.2337/db08-0303 for a high-quality digital representation of this figure.)