FIG. 2.

Insulin augments DOC2b translocation to the plasma membrane. A: 3T3-L1 adipocytes were electroporated with eGFP-DOC2b and then treated with 100 nmol/l of insulin for the times indicated. Live fluorescent images of the cells were captured by confocal microscopy. Relative fluorescences of the plasma membrane, nucleus, and cytosol were each measured in three distinct areas. At least three cells were analyzed for each condition. Results are means ± SD from three independent experiments. B: 3T3-L1 adipocytes expressing eGFP-DOC2b (WT [wild type], ΔMID, ΔC2A, or ΔC2B) were treated with or without 100 nmol/l of insulin and observed by confocal microscopy. C: 3T3-L1 adipocytes were electroporated with eGFP-DOC2b and then pretreated with 50 μmol/l of BAPTA-AM for 10 min, before treatment with insulin and observed under a confocal microscope.