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. 2009 Feb;58(2):394–402. doi: 10.2337/db08-0599

FIG. 1.

FIG. 1.

Cytotoxic responses of peptide-specific HLA-A2.1–restricted murine T-cells against preproinsulin peptides. Five to six HHD mice were immunized in vivo for each preproinsulin peptide (peptides 6–14, [A]; 6–16, [B]; 2–11, [C]; 14–23, [D]; 15–24, [E]; and 1–8, [F]). Spleen cells were restimulated in vitro 11 days later using irradiated peptide-pulsed HHD lymphoblasts. Spleen cells were then tested for cytolytic activity against HHD-transfected RMA-S targets loaded with relevant preproinsulin peptide (○) or irrelevant negative control peptide (▪) using chromium release assay. The figure represents the percentage of specific lysis (vertical axis) obtained for 1:1, 2:1, 3:1, and 4:1 effector:target (E/T) ratio (horizontal axis).