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. 2009 Feb;58(2):394–402. doi: 10.2337/db08-0599

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Copyright © 2009, American Diabetes Association

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 2. — Cytotoxicity of anti–2–11, anti–6–14, anti–14–23, and anti–15–24 CD8⁺ T-cell clones against P815 cells transfected with the HHD-encoding and the human preproinsulin genes. Individual CD8⁺ T-cell clones were tested for cytolytic activity against HHD- and preproinsulin-transfected P815 targets loaded with relevant preproinsulin peptide (circles for 2–11, rectangles for 6–14, triangles for 14–23, squares for 15–24; open symbols) or without peptide (closed symbols, respectively), using chromium release assay. The figure represents the percentage of specific lysis (vertical axis) obtained for 1:1, 2:1, and 3:1 effector:target (E/T) ratio (horizontal axis).